Molecular Biochemistry
Course Directory: Dr Stephen C. Hardies; Office: 437 D, 567-3735, hardies@uthscsa.edu
Schedule:
Spring 2007
2:00 - 4:00 Mondays and Wednesdays, in room 425D. Note schedule changes in red
Wed. 1/17/7 2 hr. CLASS CANCELLED due to weather-related closure of HSC. Dr. Stephen C.Hardies
Mon 1/22/7 2 hr. Basic PCR, Construction with PCR, Real-time PCR. notes Realtime PCR screenshot, Standard Curve. Dr. Stephen C. Hardies
Wed. 1/24/7 2 hr. Basic Enzymology   Contact information and General Reference materials for Dr. Barnes Dr. Larry D. Barnes
Mon. 1/29/7 2 hr. Basic Kinetics, including Henri-Michaelis-Menten rapid equilibrium kinetics and Briggs-Haldane steady state kinetics. Dr. Larry D. Barnes
Wed. 1/31/7 1 hr.


1 hr.
Protein-ligand binding (equilibrium analysis).


Multisite Enzymes: Kinetic modes; Use of Hill plots; Physiologic significance of multisite enzymes.
Dr. Larry D. Barnes
Mon. 2/5/7 2 hr. Multisite Enzymes: Kinetic modes; Use of Hill plots; Physiologic significance of multisite enzymes, continued Dr. Larry D. Barnes
Wed. 2/7/7 2 hr. Cloning and protein expression in E. coli, addressing problems in expression, stability, and folding. Dr. Stephen C. Hardies
Thur. 2/8/7 2 hr.  Homework problem review. 3:00 - 5:00, Rm 425D Dr. Larry D. Barnes
Mon. 2/12/7 2 hr.  Midterm exam. 3:30 - 5:30, Rm 425D Key for Dr. Barnes' questions Key for Dr. Hardies' questions
Wed. 2/14/7 2 hr. Site specific In vitro mutagenesis for the explorationm of protein function. 3:30 - 5:30, Rm 425D Dr. Stephen C. Hardies
Wed. 2/21/7 2 hr. Drosophila genetics. Slides Dr. Don McEwen
Mon. 2/26/7 2 hr. Topics in Bioinformatics Lecture Originally Scheduled on Mammalian Genetics has been postponed until Tue, March 6. Dr. Stephen C. Hardies
Wed. 2/28/7 2 hr. Microarray analysis Dr. Yousin Suh
Mon. 3/5/7 2 hr. Bacterial genetics Dr. Lee McAlister-Henn
Tue. 3/6/7 2 hr. Mammalian Genetics, Rm 425D, 2:00 - 4:00. Slides References Dr. Don McEwen
Wed. 3/7/7 2 hr. Yeast Molecular genetics Dr. Lee McAlister-Henn
week of 3/12 Spring Break
Mon. 3/19/7 2 hr.  Final Exam. Will be held in room 2.030 (under library foyer) 2:00 - 4:00. Key to final exam.