James Stockand, Professor- Department of Physiology
|Education:||B.S.,Texas A & M University, 1991|
Ph.D., Univ. of Texas Health Science Center at Houston, 1996
Cell model of a Principal cell. Aldosterone modulates ENaC in these cells by first affecting gene expression through the mineralocorticoid receptor and inducing expression of the Ki-Ras and Sgk signaling proteins, which then signal to ENaC to modulate ion channel activity.
My laboratory uses a number of contemporary methodologies, including electrophysiology, molecular biology, biochemistry, genomics and proteomics, and fluorescence microscopy to investigate regulation of ENaC and aldosterone signaling. We routinely use yeast, bacteria, immortalized cell lines and animals in this regard. Our lab is equipped to perform real-time measurement of ion channel activity and fluorescence imaging, and performs biophysical analysis of ion channel activity and gating, creates point mutations and chimeric proteins and genes, investigates cellular signal transduction, reconstitutes signaling pathways in heterologous systems, and determines the molecular mechanisms modulating gene expression, and uses the power of yeast genetics in addition to immortalized mammalian cells to investigate ENaC targeting and regulation. The pictures below give some idea of the methods we employ.
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