P. John Hart, PhD photo
P. John Hart, PhD

P. John Hart, PhD, Ewing Halsell-Presidents Council Distinguished Professor

Web Page(s):http://hartlab.uthscsa.edu; http://xray.uthscsa.edu
Education:B.A. Chemistry, University of Kansas 1987

B.A. Psychology, University of Kansas 1988

Ph.D. Biochemistry, University of Texas at Austin 1993

Post Doctoral:Structural Biology, UCLA 1994-1998
Cross Appointments:Department of Veterans Affairs, South Texas Veterans Health Care System
Other Faculty Positions:Director
X-ray Crystallography Core Laboratory
Awards and Academic Honors:Ewing Halsell, Presidents Council Distinguished Professorship of Biochemistry, 2006-present

San Antonio Business Journal "Health Care Heroes" Award, 2004

Presidential Junior Research Scholar, UTHSCSA, 2003

CREF New Faculty Award, UTHSCSA, 1999-2001

Howard Hughes Medical Institute New Faculty Award, UTHSCSA, 1998-1999

U.S. Dept. of Energy Alexander Hollaender Distinguished Postdoctoral Fellow, UCLA, 1994-1996

Eakin Award for Excellence in Graduate Research in Biochemistry, UT Austin, 1992

NIH Predoctoral Fellow, UT Austin, 1990-1993

Robert A. Welch Predoctoral Fellow in Chemistry, UT Austin, 1988-1990

Baseball Academic All-Big 8 Conference, University of Kansas, 1988-1990

Personal Statement:We usesingle crystal X-ray diffraction, together with other biophysical methods, to characterize the structure and action of biological macromolecules important to human health. There are multiple research foci in my laboratory, and several of these reflect our long-standing interest in metalloproteins. We are also interested in bacterial pathogenisis and we seek mechanisms to combat parasites that infect humans. A more recent interest is structure-based drug design. I serve as Director of the X-ray Crystallography Core Laboratory at UTHSCSA (http://xray.uthscsa.edu). The mission of the X-ray Core Lab is to bring structure determination technology to all researchers so that they may obtain structures of their own proteins of interest.

Research Interest:

1) AQ-1399 (Hart) 06/01/13-5/31/16
Robert A. Welch Foundation
"Structural and Chemical Profiling of the Sulfotransferase of Schistosoma"
The major goals of this research project are to determine the three-dimensional structure of wild type and oxamniquine-resistant variants of SmULT (Schistosoma mansoni SULfoTransferase).
Role: PI
Collaboration with LoVerde and Anderson Labs

2) 5 U19 AI070412/(Baseman/Hart Proj. 3) NIH-NIAID 08/01/11-07/31/16
"Structure and Action of CARDS Toxin"
The goals of this research project are to understand the structure/function relationships in community acquired respiratory distress syndrome toxin from Mycoplasma pneumoniae.
Role: PI Subproject 3

3) VA 1I01BX000506-01 (Hart)07/01/10-06/30/14
Biomedical Laboratory Research & Development
"Nascent SOD1 in Amyotrophic Lateral Sclerosis"
The major goals of this research project are to understand the role of metal-deficient/disulfide-reduced pathogenic SOD1 mutants.
Role: PI

4) William and Ella Owens Medical Research Foundation 01/01/11-12/31/13
"X-ray Crystal Structures of Matrix Metalloproteinase 13 (MMP-13) in Complex with Exosite Inhibitors"
The goal of this translational project is to identify and develop inhibitors specific for MMP-13 over other MMPs for use as therapeutic agents to treat osteoarthritis and human melanoma.
Role: PI
Collaboration with the Fields Lab, Torrey Pines Institute, Florida

5) W911NF-11-1-0316 DOD Center of Excellence 04/01/11-03/31/16
Department of Defense
"Center of Excellence in Infection Genomics"
The goal of this project is to educate undergraduate and graduate students in the tools of microbiology and structural biology to broad their perspectives while simultaneously working on important problems with regard to human health.
Role: Director, structural biology component

Please see my publication list.

image1 image2
The Chlamydia trachomatis secreted immunodominant antigen Pgp3. Left: Oxamniquine (yellow) bound to the sulfotransferase from the human parasite Schistosoma mansoni. Right: The worm wrapped around the sulfotransferase structure.

Complete Publication Listing

Lab Associations:
  Xiaohang Cao, MD, Senior Research Associate
  Stephen Holloway, PhD, Senior Research Associate
  Alex Taylor, PhD, Senior Research Scientist

Graduate Students:
  Argentina Becker, Molecular Biophysics & Biochemistry

  Nathaniel Clark, PhD, Hart Lab

Lab Alumni:
  Ahmad Galaleldeen, PhD, Hart Lab