PROGRAM: T-COFFEE (Version_2.11) -run_name S [1] /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598 -mem_mode S [0] mem -extend D [1] 1 -extend_mode S [0] very_fast_triplet -max_n_pair D [0] 10 -seq_name_for_quadruplet S [0] all -compact S [0] default -clean S [0] no -do_self FL [0] 0 -do_normalise D [0] 1000 -seq S [0] -in S [1] /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.in0 Mlalign_id_pair Mslow_pair Msap_pair Mfugue_pair -pdb S [0] -out_lib W_F [1] no -outseqweight W_F [0] no -seq_to_align S [0] -cosmetic_penalty D [0] -50 -gapopen D [0] 0 -gapext D [0] 0 -fgapopen D [0] 0 -fgapext D [0] 0 -nomatch D [0] 0 -newtree W_F [0] default -usetree R_F [0] -tree_mode S [0] slow -quicktree FL [0] 0 -outfile W_F [0] default -maximise FL [1] 1 -output S [1] clustalw_aln score_pdf fasta_aln score_ascii score_html -infile R_F [0] -matrix S [0] -tg_mode D [0] 1 -profile_mode S [0] cw_profile_profile -profile_comparison S [0] full50 -dp_mode S [0] cfasta_pair_wise -ktuple D [0] 1 -ndiag D [0] 0 -diag_threshold D [0] 0 -diag_mode D [0] 0 -sim_matrix S [0] vasiliky -type S [0] -outorder S [1] input -seqnos S [1] on -case S [1] lower -cpu D [0] 0 -maxnseq D [1] 50 -maxlen D [1] 2000 -weight S [0] sim -seq_weight S [0] no -align FL [1] 1 -mocca FL [0] 0 -start D [0] 0 -len D [0] 0 -scale D [0] 0 -mocca_interactive FL [0] 0 -evaluate_mode S [1] t_coffee_slow -get_type FL [0] 0 -clean_aln D [0] 0 -clean_threshold D [1] 1 -clean_iteration D [1] 1 -clean_evaluate_mode S [0] t_coffee_fast -profile S [0] -profile1 S [0] -profile2 S [0] -extend_matrix FL [0] 0 -prot_min_sim D [40] 40 -prot_max_sim D [60] 60 -prot_min_cov D [0] 0 -pdb_min_sim D [30] 30 -pdb_max_sim D [100] 100 -pdb_min_cov D [50] 50 -pdb_blast_server W_F [0] SIB -prot_blast_server W_F [0] SIB -pdb_db W_F [0] nrl3d -protein_db W_F [0] nr -method_log W_F [0] no -struc_to_use S [0] -cache W_F [1] no -align_pdb_param_file W_F [0] no -align_pdb_hasch_mode W_F [0] hasch_ca_trace_bubble -msa_mode S [0] tree -lalign_n_top D [0] 10 -iterate D [0] 0 -trim D [0] 0 -split D [0] 0 -trimfile S [0] default -split D [0] 0 -split_nseq_thres D [0] 0 -split_score_thres D [0] 0 -check_pdb_status D [1] 1 -seq_to_keep S [0] INPUT FILES Input File (S) /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.in0 Format fasta_seq Input File (M) lalign_id_pair Input File (M) slow_pair Input File (M) sap_pair Input File (M) fugue_pair INPUT SEQUENCES: 4 SEQUENCES [PROTEIN] Input File /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.in0 Seq 1ajsA Length 365 type PROTEIN Struct Yes PDBID 1AJS Input File /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.in0 Seq 2dkb Length 380 type PROTEIN Struct Yes PDBID 2DKB Input File /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.in0 Seq 2gsaA Length 365 type PROTEIN Struct Yes PDBID 2GSA Input File /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.in0 Seq 1ax4A Length 387 type PROTEIN Struct Yes PDBID 1AX4 READ/MAKE LIBRARIES:[5] lalign_id_pair [method] [TOT= 6][ 0 %] [TOT= 6][ 33 %] [TOT= 6][ 50 %] [TOT= 6][ 66 %] [TOT= 6][ 83 %] [TOT= 6][100 %] slow_pair [method] [TOT= 6][ 0 %] [TOT= 6][ 33 %] [TOT= 6][ 50 %] [TOT= 6][ 66 %] [TOT= 6][ 83 %] [TOT= 6][100 %] sap_pair [method] [TOT= 6][ 0 %] [TOT= 6][ 33 %] [TOT= 6][ 50 %] [TOT= 6][ 66 %] [TOT= 6][ 83 %] [TOT= 6][100 %] fugue_pair [method] [TOT= 6][ 0 %] [TOT= 6][ 33 %] [TOT= 6][ 50 %] [TOT= 6][ 66 %] [TOT= 6][ 83 %] [TOT= 6][100 %] Library Total Size: [4797] UN-WEIGHTED MODE: EVERY SEQUENCE WEIGHTS 1 COMPUTE PAIRWISE SIMILARITY [dp_mode: cfasta_pair_wise] [tree_mode: slow] 1ajsA 1ax4A identity= 9% score= 30 1ajsA 2dkb identity= 9% score= 31 1ajsA 2gsaA identity= 8% score= 31 1ax4A 2dkb identity= 11% score= 28 1ax4A 2gsaA identity= 12% score= 29 2dkb 2gsaA identity= 20% score= 40 MAKE NEIGHBOR JOINING DENDROGRAM [MODE=slow][DONE] PROGRESSIVE_ALIGNMENT [Tree Based] Group 5: [Group 3 ( 1 seq)] with [Group 4 ( 1 seq)]-->[Score= 17][Len= 389] Group 6: [Group 5 ( 2 seq)] with [Group 1 ( 1 seq)]-->[Score= 30][Len= 417] Group 7: [Group 2 ( 1 seq)] with [Group 6 ( 3 seq)]-->[Score= 42][Len= 444] OUTPUT RESULTS #### File Type= TREE Format= newik Name= /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.dnd #### File Type= MSA Format= clustalw_aln Name= /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.clustalw_aln #### File Type= MSA Format= score_pdf Name= /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.score_pdf #### File Type= MSA Format= fasta_aln Name= /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.fasta_aln #### File Type= MSA Format= score_ascii Name= /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.score_ascii #### File Type= MSA Format= score_html Name= /usr/local/httpd/htdocs/Tcoffee/Tmp/tcfAA99504_23598.score_html T-COFFEE Memory Usage: Current= 3.929 Mb, Max= 6.426 Mb T-COFFEE CPU Usage: 8150 millisec