Calibration value table

Calibration values:

Avance600

operator/sample specifics ¹H ¹⁵N
on ¹⁵N urea standard ¹³C
on ¹³C acetate standard

6/19/02 was when it was first noticed that the ¹H channel was requiring 4-5 lower dB

Steve Hardies
Ubiquitin standard
50 mM KP_i, pH 5.8
T=299K HNCACBSE (7/1/02)
8.34u @ -6dB (P1@PL1)
Don't use -6 dB in future
2080u @ 36dB (P10@sp1,
with seduce1.jc)
40.1u @ 8dB (Pll@PLll and pcpd1 with waltz16) HNCACBSE (6/19/02)
T=299K
42.3u; -5 dB (P2@PL2)
181u @7.9dB (pcpd2 @PL12 with waltz16) HNCACBSE (6/19/02)
T=299K
14.3u; -3 dB (P3 @PL3)
299u @10.83 dB (pcpd3 @ sp3 with seduce1c6)
249u @9.35 dB (pcpd3 @ sp3 with seduce1c5 calibrated but not used)

Steve Hardies HSQC (6/19/02)
T=299K
45u @-4.4dB (P7@PL7)
181u @7.9dB(pcpd3@PL3)
Calibrated, not tested HSQC (6/19/02)
T=299K
23.7u @1.45dB (P5@PL5)
Calibrated, not tested

7/17/02 Andy remade coretab; this may slightly alter hydrogen pulse times.

Steve Hardies
L1, p190
50 mM NaCl, 30 mM NaP_i
pH 5.79, T=300K 7/24/02
Series of calibrated square pulses to test coretab linearity
7.95u @ -6dB
8.88u @ -5dB
16u @ 0 dB
40.7u @ 8dB
173u @ 20dB
1085u @ 36 dB
1718 u @ 40 dB

Steve Hardies
L1,p190, 50 mM NaCl,
30 mM NaP_i,
pH=5.79, T=300K HNCACBSE (7/24)
8.88u @-5dB (P1@PL1)
2056u @35dB(P10@sp1,
with seduce1.jc)
40.7u @8dB (Pll@PLll and pcpd1 with waltz16)

Steve Hardies
L1, p190 50 mM NaCl,
30 mM NaP_i; T=300K HSQC (7/29)
8.85 @ -5 dB(P1@PL1)
1020u @35 dB (P2@PL2)
2040u @35 dB(p3@sp0 with seduce1.jc)
This set done fairly crudely.

Andy Hinck
Ubiquitin standard in
50 mM KP_i, pH 5.8
T=300K CBCACONHSE (7/30)
Wisconsin version
8.5 @ -5.1 dB (P1@PL1)
50u @10 dB (pcpd1@PL11, cpdprg1=dipsi2) CBCACONHSE (7/30)
T=300, Wisconsin version
45u @-4.4dB(P2 @PL2)
180u@7.9 dB (pcpd2 @PL12, cpdprg2= waltz16)
CBCACONHSE (7/30)
T=300, Wisconsin version
48.2@7.7 dB (p3@PL3 )
21.6u@0.8 dB (P6@PL6)
53.5u @ 8.6 dB (P4@PL4)
250u@9.4 dB (P5@sp5 seduce1.jc spoffs5 20068 )
53.5u@8.5dB (p7@sp7 spnam7=rect32 spoffs7=18107)
47.8u @1.4dB (p8@sp8 spnam8=rect32 spoffs8=18107)
125u@9.4 dB (pcpd3@sp3 spname3=seduce1.jc spoffs3 = 0; cpdprg3=waltz16sp3)

Steve Hardies, L1 p190
50 mM NaCl, 30 mM NaP_i
pH 5.79, T=300 CBCACONHSE (7/31)
Wisconsin version
8.85u @ -5 dB (P1@PL1 )
47.93u @9.5 dB (pcpd1@PL11)

Andy Hinck
Ubiquitin standard in 50 mM KP_i, pH 5.8, T=300K CBCACONH (7/30)
NIH version
8.5u@-5.1 dB (P1@PL1)
1055u @ 36 dB (P2@PL2)
50u@10 dB (P30@PL30)

CBCACONH (7/30)
NIH version , T=300
40@-5 dB (P17@PL17)
170@7.9dB (P31@PL31) CBCACONH (7/30)
NIH version, T=300
47.9u@7.6dB (P4@PL4)
53u@8.5 dB (P5@PL5)
21.4u@0.7 dB(P6@PL6)
47.4u @1.4 dB (p7 @sp1, spnam7= rect32, spoffs7=-18257)
300u @12.9dB (p8@sp0;spnam0= sinc1.0; spoffs0 = +20219)

Steve Hardies, L1 p190
50 mM NaCl, 30 mM NaP_i
pH 5.79, T=300 CBCACONH (7/31)
NIH version
8.85u @-5 dB ( P1@PL1)
1035u@ 35.5 dB (P2@PL2)
47.93u@9.5dB(P30@PL30)

Avance500

Operator/sample specifics ¹H ¹⁵N ¹³C

Maria
Sample in 50 mM NaP_i
pH 6.0 HSQC (7/16/02)
8.45u @ -2dB (P1@PL1)
1020u @ 39dB (P2@PL2) HSQC (7/16/02)
45u @-3.1dB (P7@PL7)
200u @10dB (pcpd3@PL3) HSQC (7/16/02)
28.7u @2.35dB (P5@PL5)

Steve Hardies
Sample in 50 mM NaCl,
30 mM NaP_i, pH 5.79 HSQC (7/21/02)
9.45u @ -2dB (P1@PL1)
1025u @ 38.5dB(P2@PL2)
2040 @ 38 dB (P3@sp0, with seduce1.jc)

operator/sample specifics	¹H	¹⁵N on ¹⁵N urea standard	¹³C on ¹³C acetate standard

	6/19/02 was when it was first noticed that the ¹H channel was requiring 4-5 lower dB

Steve Hardies Ubiquitin standard 50 mM KP_i, pH 5.8 T=299K	HNCACBSE (7/1/02) 8.34u @ -6dB (P1@PL1) Don't use -6 dB in future 2080u @ 36dB (P10@sp1, with seduce1.jc) 40.1u @ 8dB (Pll@PLll and pcpd1 with waltz16)	HNCACBSE (6/19/02) T=299K 42.3u; -5 dB (P2@PL2) 181u @7.9dB (pcpd2 @PL12 with waltz16)	HNCACBSE (6/19/02) T=299K 14.3u; -3 dB (P3 @PL3) 299u @10.83 dB (pcpd3 @ sp3 with seduce1c6) 249u @9.35 dB (pcpd3 @ sp3 with seduce1c5 calibrated but not used)

Steve Hardies		HSQC (6/19/02) T=299K 45u @-4.4dB (P7@PL7) 181u @7.9dB(pcpd3@PL3) Calibrated, not tested	HSQC (6/19/02) T=299K 23.7u @1.45dB (P5@PL5) Calibrated, not tested

	7/17/02 Andy remade coretab; this may slightly alter hydrogen pulse times.

Steve Hardies L1, p190 50 mM NaCl, 30 mM NaP_i pH 5.79, T=300K	7/24/02 Series of calibrated square pulses to test coretab linearity 7.95u @ -6dB 8.88u @ -5dB 16u @ 0 dB 40.7u @ 8dB 173u @ 20dB 1085u @ 36 dB 1718 u @ 40 dB

Steve Hardies L1,p190, 50 mM NaCl, 30 mM NaP_i, pH=5.79, T=300K	HNCACBSE (7/24) 8.88u @-5dB (P1@PL1) 2056u @35dB(P10@sp1, with seduce1.jc) 40.7u @8dB (Pll@PLll and pcpd1 with waltz16)

Steve Hardies L1, p190 50 mM NaCl, 30 mM NaP_i; T=300K	HSQC (7/29) 8.85 @ -5 dB(P1@PL1) 1020u @35 dB (P2@PL2) 2040u @35 dB(p3@sp0 with seduce1.jc) This set done fairly crudely.

Andy Hinck Ubiquitin standard in 50 mM KP_i, pH 5.8 T=300K	CBCACONHSE (7/30) Wisconsin version 8.5 @ -5.1 dB (P1@PL1) 50u @10 dB (pcpd1@PL11, cpdprg1=dipsi2)	CBCACONHSE (7/30) T=300, Wisconsin version 45u @-4.4dB(P2 @PL2) 180u@7.9 dB (pcpd2 @PL12, cpdprg2= waltz16)	CBCACONHSE (7/30) T=300, Wisconsin version 48.2@7.7 dB (p3@PL3 ) 21.6u@0.8 dB (P6@PL6) 53.5u @ 8.6 dB (P4@PL4) 250u@9.4 dB (P5@sp5 seduce1.jc spoffs5 20068 ) 53.5u@8.5dB (p7@sp7 spnam7=rect32 spoffs7=18107) 47.8u @1.4dB (p8@sp8 spnam8=rect32 spoffs8=18107) 125u@9.4 dB (pcpd3@sp3 spname3=seduce1.jc spoffs3 = 0; cpdprg3=waltz16sp3)
Steve Hardies, L1 p190 50 mM NaCl, 30 mM NaP_i pH 5.79, T=300	CBCACONHSE (7/31) Wisconsin version 8.85u @ -5 dB (P1@PL1 ) 47.93u @9.5 dB (pcpd1@PL11)
Andy Hinck Ubiquitin standard in 50 mM KP_i, pH 5.8, T=300K	CBCACONH (7/30) NIH version 8.5u@-5.1 dB (P1@PL1) 1055u @ 36 dB (P2@PL2) 50u@10 dB (P30@PL30)	CBCACONH (7/30) NIH version , T=300 40@-5 dB (P17@PL17) 170@7.9dB (P31@PL31)	CBCACONH (7/30) NIH version, T=300 47.9u@7.6dB (P4@PL4) 53u@8.5 dB (P5@PL5) 21.4u@0.7 dB(P6@PL6) 47.4u @1.4 dB (p7 @sp1, spnam7= rect32, spoffs7=-18257) 300u @12.9dB (p8@sp0;spnam0= sinc1.0; spoffs0 = +20219)
Steve Hardies, L1 p190 50 mM NaCl, 30 mM NaP_i pH 5.79, T=300	CBCACONH (7/31) NIH version 8.85u @-5 dB ( P1@PL1) 1035u@ 35.5 dB (P2@PL2) 47.93u@9.5dB(P30@PL30)

Operator/sample specifics	¹H	¹⁵N	¹³C

Maria Sample in 50 mM NaP_i pH 6.0	HSQC (7/16/02) 8.45u @ -2dB (P1@PL1) 1020u @ 39dB (P2@PL2)	HSQC (7/16/02) 45u @-3.1dB (P7@PL7) 200u @10dB (pcpd3@PL3)	HSQC (7/16/02) 28.7u @2.35dB (P5@PL5)

Steve Hardies Sample in 50 mM NaCl, 30 mM NaP_i, pH 5.79	HSQC (7/21/02) 9.45u @ -2dB (P1@PL1) 1025u @ 38.5dB(P2@PL2) 2040 @ 38 dB (P3@sp0, with seduce1.jc)