This is a repeat of the HNCACB on the p190 L1 sample using the NIH pulse program on the 700
MHz instrument.

Andy did temperature calibration, and it became clear that there was a 2.5 degree discrepency between the ethylene glycol indicated temperature and the DSS/H2O indicated temperature.  I suspect that there is water in the ethylene glycol sample accumulating over time from the lab humidity.

Although it might be more comparable to do 302.5 K, setting 300K is consistent with the DSS-H2O result.  I'm going to try the lower temperature to see if it cleans up the spectrum any.

The probe with atmm is having a problem, so we are using the manually tuned one.

;Proton settings - Set carrier to H2O
;p1    -  square 90 1H @ pl1, for excitation, typically p1 = 7.9u @ pl1 = -2dB
;p2    -  square 90 1H @ pl1, for watergate, typically p2 = 1000u @ pl2 = +39.6d
B
;p3    -  shaped 90 1H @ sp0, for water flipback, typically p3 = 2m @ sp0 = +39.
2 dB
;         "spnam0 seduce1.jc", "spoffs0 0"
;pcpd1  -  square 90 1H @ pl30, for excitation, typically pcpd1 = 50u @ pl30 = +
13.2dB
;         "cpdprg1 dipsi2"